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NFAT-Luciferase


by Greg Gurda
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Description (including epitope tag if present)

NFAT-luciferase reporter (9 multimerized NFAT binding sites upstream of a minimal TATA-containing promoter fused to luciferase) was cloned into the multiple cloning site of pAC-CMV-pLpA construct. The resulting plasmid was cotransfected into HEK293 cells with plasmid pJM17 which contains the Ad5 genome.  

Source

How used

Optimized and performed in isolated, primary mouse acinar cells as well as secondary cell lines includingacinar-derived AR42J, 266-6 and non-acinar HEK293 and NIH 3T3.  Please refer to methods section in pancreapedia “Quantitative assays of transcriptional activity:  NFAT-Luciferase reporter”.

Publications describing the vector and usage

  1. Cholecystokinin activates pancreatic calcineurin-NFAT signaling in vitro and in vivo. Gurda GT, Guo L, Lee SH, Molkentin JD, Williams JA.  Mol Biol Cell. 2008 Jan;19(1):198-206.                                      
  2. Regulator of calcineurin 1 controls growth plasticity of adult pancreas.  Gurda GT, Crozier SJ, Ji B, Ernst SA, Logsdon CD, Rothermel BA, Williams JA.  Gastroenterology. 2010 Aug;139(2):609-19