Rab 3D N135I

Full name (if applicable): 

HA-tagged mouse Rab3D with N135I mutation in pAdEasy-1

Description (including epitope tag if present): 

Prepared using pAdtrack-CMV shuttle vector. The vector also expresses EGFP under a separate promoter.


Prepared by submitter

How used: 

 Used to infect mouse pancreatic acini in overnight suspension culture.  The amount of protein expressed and its localiztion can be followed by immunoblotting and Immunohistochemistry with antibody against the HA tag.

Publications describing the vector and usage: 

J Biol. Chem 277:18002-18009 2002; J Biol Chem 278: 50053-50060, 2003