Description (including epitope tag if present):
N-terminal HA tagged human RGS2 from the University of Missouri Rollo was subcloned into the Adtrack-CMV vector and recombined with pAdEasy-1 in BJ5183 strain of E. coli.
Source:
Constructed by Patrick J Casey, Duke University. Available from Dr. Williams with permission from Dr. Casey
How used:
Has been used to inhibit Gq signaling in prostate cancer cells and mouse pancreatic acinar cells. Does not affect GaS or Ga13
Publications describing the vector and usage:
- Kelly, P, LN Stemmle, JF Madden, TA Fields, Y Daaka, and PJ Casey. A role for the G12 family of heterotrimeric G proteins in prostate cancer invasion. J Biol Chem 281:26483-26490, 2006
- Sabbatini, ME, Y Bi, B Ji, SA Ernst, and JA Williams. CCK activates RhoA and Rac1 differentially through Ga13 and Gaq in mouse pancreatic acini. Am J Physiol Cell Physiol. 298:C592-C601, 2010.